FLIMera
SPAD array imaging camera for dynamic FLIM studies at real time video rates
Widefield TCSPC FLIM camera based on 192x128 SPAD pixel array with in-pixel TDC technology. Timing resolution 50ps/bin nominal. Includes EzTime-Image software. Compatible with a high repetition rate pulsed laser illumination sources (e.g. DeltaDiode).
Features:
- TCSPC per pixel architecture offers 24,576 simultaneous decays
- Complex multicomponent lifetimes
- Decay kinetics are acquired directly.
- Sample analysis with up to 5 exponential decays
- EzTime Image simple and efficient software interface
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Perfect for complex and sophisticated molecular analysis like live cell dynamics and detection of biomarkers for disease diagnostics.
Modes of operation:
The FLIMera is designed for use with HORIBA’s highly intuitive EzTime Image software. This software is used for FLIMera control, data acquisition and analysis. FLIMera provides 4 modes of operation.
- Manual – User start and stop of acquisition, providing TCSPC intensity image and FLIM data for lifetime analysis.
- Timed – User defined run time for acquisition, providing TCSPC intensity image and FLIM data for lifetime analysis.
- Streaming to HDF5 file – TCSPC data can be streamed, for a user-determined time period, to a HDF5 file. This contains full records for each photon in every pixel, so no data is lost, and can be used to reconstruct frames for full decay analysis.
- Phasor Plot – Complementing the histogram representation is the ability to show and select lifetime data in the form of a phasor plot in real time, as seen in Figure 3. The phasor plot requires initial calibration using a lifetime standard.
Compatibility:
If you want more information about Fluorescence Spectroscopy please visit our website on What is Fluorescence Spectroscopy? and FLIMera SPAD array imaging camera for dynamic FLIM studies at real time video rates - HORIBA
