Check for Cracks or Damage: Regularly inspect the tube for cracks, especially around the cap connection points where stress is highest. Similarly, check the cap for any signs of wear, including cracks or loss of its sealing ability.
Cap Sealing Surface: Ensure that the sealing surface inside the cap is not worn or damaged, as this could result in improper sealing, which could lead to leaks or contamination.
Debris Check: Periodically check the tube for any blockages that may restrict sample flow, especially if working with samples containing particulates.