FLIMera

SPAD array imaging camera for dynamic FLIM studies at real time video rates

Widefield TCSPC FLIM camera based on 192x128 SPAD pixel array with in-pixel TDC technology. Timing resolution 50ps/bin nominal. Includes EzTime-Image software. Compatible with a high repetition rate pulsed laser illumination sources (e.g. DeltaDiode).

Features:

TCSPC per pixel architecture offers 24,576 simultaneous decays
Complex multicomponent lifetimes
Decay kinetics are acquired directly.
Sample analysis with up to 5 exponential decays
EzTime Image simple and efficient software interface
Perfect for complex and sophisticated molecular analysis like live cell dynamics and detection of biomarkers for disease diagnostics.

Modes of operation:

The FLIMera is designed for use with HORIBA’s highly intuitive EzTime Image software. This software is used for FLIMera control, data acquisition and analysis. FLIMera provides 4 modes of operation.

Manual – User start and stop of acquisition, providing TCSPC intensity image and FLIM data for lifetime analysis.
Timed – User defined run time for acquisition, providing TCSPC intensity image and FLIM data for lifetime analysis.
Streaming to HDF5 file – TCSPC data can be streamed, for a user-determined time period, to a HDF5 file. This contains full records for each photon in every pixel, so no data is lost, and can be used to reconstruct frames for full decay analysis.
Phasor plot – Complementing the histogram representation is the ability to show and select lifetime data in the form of a phasor plot in real time, as seen in Figure 3. The phasor plot requires initial calibration using a lifetime standard.