Description
Discover New Horizons for your Fluorescence Spectrometer: Unleashing the Potential of our Absorption Accessory
The absorption accessory makes the QuantaMaster™ fluorometer much more versatile and user friendly. It eliminates the need for a separate and often expensive UV-VIS spectrophotometer.
Specifications:
- Sample Holder: 10 x 10 mm cuvette holder
- Minimum Absorbance: 0.005
- Maximum Absorbance: 3.00
- Mode of Operation: Single beam
The QuantaMaster™ fluorometers have been recognized for their high sensitivity and the ability to adapt to various applications due to their modular design. The addition of the simple absorption accessory enhances the performance and versatility of the instrument even more.
In many fluorescence experiments it is necessary to know the absorption properties of the sample. The ABS-ACC absorption accessory fits directly into a cuvette sample holder and enables the user to measure the absorption spectrum or check the optical density of the sample without reconfiguring the QuantaMaster™ fluorometer.
Absorption measurements are complementary to fluorescence. They are necessary for fluorescence quantum yield determination and more accurate results are usually obtained by using the same optical path for both fluorescence and absorption measurements. The ability to check the optical density during a fluorescence experiment makes it easy to avoid inner filter effects. By comparing the absorption and excitation spectra one can draw conclusions about the purity of the sample.
Proper maintenance and timely replacement of parts are essential to ensure accurate results and the longevity of the accessory.
Maintenance and replacement:
- Clean cuvettes, lenses, and mirrors regularly to prevent dust and residue buildup.
- Keep the sample compartment clean and free from spills.
- Routinely inspect the accessory for any signs of wear or damage.
- Check cables, connectors, and other electronic components for integrity.
- Look for any loose parts or screws and tighten them as needed.
- Ensure that the optical path is properly aligned.
- Periodically calibrate the accessory to ensure measurement accuracy.
Inexpensive and easy-to-use accessory to achieve quality absorbance on HORIBA fluorometers
ABS-ACC, perfect to avoid Inner Filter Effect:
- The fluorescence intensity is proportional to the intensity of the light source (actually proportional to the absorbed light intensity).
- The linear relationship between fluorescence intensity and concentration holds only for dilute solutions where absorbance is lower than ~ 0.1 OD.
- At high concentrations, light absorption can make the measured fluorescence intensity lower than the true fluorescence emission: This is called the Inner Filter Effect (IFE).
- As a consequence, IFE limits the linearity of the fluorescence signal at higher sample concentrations due to primary and secondary re-absorption of fluorescence.
- The ABS-ACC accessory is the perfect tool to monitor the concentration of the samples to avoid the inner filter effect by easily determining if the absorbance is too high.
The ABS-ACC absorption accessory requires one of the following types of sample holder trays.
- Standard room-temperature cuvette holder
- Single position water jacketed cuvette holder
- Single position thermo-electric cuvette holder
Compatibility:
If you want more information about this topic, please visit our website on What is Fluorescence Spectroscopy?
